Quantitative calculation of concentration values using a single standard.
This method first calculates the corrected absorbance by subtracting the average of the Blank wells from all samples. A standard curve is constructed by plotting the specified concentration value of the single standard against its corrected absorbance with a straight line through zero. The line is used to calculate concentrations of samples with any dilution factors applied.
If any sample is greater than the standard it is highlighted in yellow and should be re-assayed.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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