Single Standard ELISA

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Quantitative calculation of concentration values using a single standard.

This method first calculates the corrected absorbance by subtracting the average of the Blank wells from all samples. A standard curve is constructed by plotting the specified concentration value of the single standard against its corrected absorbance with a straight line through zero. The line is used to calculate concentrations of samples with any dilution factors applied.

If any sample is greater than the standard it is highlighted in yellow and should be re-assayed.



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After reviewing other analysis parameters press the Calculate button below to process your results.



Standard Concentration


Dilution Factors


Sample IDs


Run Notes