Quantitative analysis of samples using a Five Parameter Logistic (5PL) curve fit suitable for calculating concentrations from symmetrical sigmoidal calibrators. Data points are weighted using the expresson 1/y meaning that points with a lower signal have a higher weight. This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit). The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 5PL is made through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below than the lower asymptote) are highlighted in yellow.

Quantitative analysis of samples using a Five Parameter Logistic (5PL) curve fit suitable for calculating concentrations from symmetrical sigmoidal calibrators. Data points are weighted using the expresson 1/y² meaning that points with a lower signal have a higher weight. This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit). The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 5PL is made through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below than the lower asymptote) are highlighted in yellow.

Quantitative analysis of samples using a Five Parameter Logistic (5PL) curve fit suitable for calculating concentrations in accordance with Abcam's Erythropoietin Human ELISA kit. This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit). The standard data points (concentration vs. measurement) are plotted on log-log axes and a weighted 5PL is fit through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

Detection of cynomolgus TNF alpha using HTRF (an alternative to ELISA) Cisbio kit 62CTNFAPEG. TNF alpha, mainly produced by activated macrophages, is one of the main cytokines in acute phase reaction. Depending on the cellular target and the biological context, TNF alpha can activate pro-apoptosis or pro-survival pathways, thus regulating tissue development through apoptosis control. Outside of Oncology and Inflammation, TNF alpha contributes to the development of Type II Diabetes by increasing insulin resistance and modulating fatty acid metabolism. Cisbio's Cynomolgus TNF alpha kit is a homogeneous, add and read assay to measure TNF alpha secretion in supernatant. It offers a fast, efficient alternative to ELISA. This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows: 1. Delta Ratio is computed for each sample using the zero standard (Zero). 2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. 3. The %CV and Standard Deviation are calculated for each replicated sample.

Detection of human CCL2 using HTRF (an alternative to ELISA) Cisbio kit 62HCCL2PEG. Also called MCP-1 (monocyte chemoattractant protein 1), CCL2 is a chemokine mainly secreted by monocytes, macrophages and dendritic cells. Its main role is to attract monocytes, basophils and T cells. CCL2 is involved in pathologies involving monocytic infiltrates like psoriasis or atherosclerosis. In the brain, CCL2 is released by neurons, astrocytes and microglia and is part of the neuroinflammatory response observed in epilepsy, ischemia or Alzheimer’s disease. CCL2 is also secreted by adipocytes and can interfere with insulin signaling, thus suggesting a role in the inflammation linked to Diabetes. Cisbio's Human CCL2 kit is a homogeneous, add and read assay to measure CCL2 secretion in supernatant. It offers a fast, efficient alternative to ELISA. This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows: 1. Delta Ratio is computed for each sample using the zero standard (Zero). 2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. 3. The %CV and Standard Deviation are calculated for each replicated sample.

Detection of human CCL20 using HTRF (an alternative to ELISA) Cisbio kit 62HCC20PEG. Also called LARC (liver activation-regulated chemokine) or MIP3A (macrophage inflammatory protein 3), CCL20 is a chemokine mainly present in peripheral blood lymphocytes, lymph nodes and liver. CCL20 secretion is upregulated by microbial factors and inflammatory cytokines like TNFa or IFNg. Its main role is to attract lymphocytes and dendritic cells to the site of infection. In CNS, CCL20 released by endothelial cells has been shown to attract TH17 cells and promote their infiltration to the lumbar spinal cord. Cisbio's Human CCL20 kit is a homogeneous, add and read assay to measure CCL20 secretion in supernatant. It offers a fast, efficient alternative to ELISA. This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows: 1. Delta Ratio is computed for each sample using the zero standard (Zero). 2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. 3. The %CV and Standard Deviation are calculated for each replicated sample.

Detection of human CCL4 using HTRF (an alternative to ELISA) Cisbio kit 62HCCL4PEG. Also known as MIP-1beta (macrophage inflammatory protein 1 beta), CCL4 is a chemokine secreted by activated leukocytes, lymphocytes, endothelial and muscle cells in response to microbial infection but also ischemia or asthma. Cisbio's Human CCL4 kit is a homogeneous, add and read assay to measure CCL4 secretion in supernatant. It offers a fast, efficient alternative to ELISA. This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows: 1. Delta Ratio is computed for each sample using the zero standard (Zero). 2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. 3. The %CV and Standard Deviation are calculated for each replicated sample.

Detection of human CXCL1 using HTRF (an alternative to ELISA) Cisbio kit 62HCXC1PEG. Also called GROa, KC NAP-3 (neutrophil activating protein 3) or MSGA-alpha (melanoma growth stimulating activity alpha), CXCL1 is a member of the CXC chemokine family. Mainly expressed by macrophages, CXCL1 attracts neutrophils and is also involved in angiogenesis, inflammation and tumorigenesis. Cisbio's Human CXCL1 kit is a homogeneous, add and read assay to measure CXCL1 secretion in supernatant. It offers a fast, efficient alternative to ELISA. This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows: 1. Delta Ratio is computed for each sample using the zero standard (Zero). 2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. 3. The %CV and Standard Deviation are calculated for each replicated sample.

Detection of human CXCL10 using HTRF (an alternative to ELISA) Cisbio kit 62HCX10PEG. Also called IP-10 (interferon gamma-induced protein 10) or small-inducible cytokine B10, CXCL10 is a chemokine secreted by monocytes, fibroblasts and endothelial cells in response to IFN gamma. CXCL10's main effect is to promote cell migration through Calcium signaling. Target cells include macrophages, T cells, NK cells and dendritic cells. CXCL10 has significant anti-tumor effects as well as anti-angiogenic effects. Cisbio's Human CXCL10 kit is a homogeneous, add and read assay to measure CXCL10 secretion in supernatant. It offers a fast, efficient alternative to ELISA. This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows: 1. Delta Ratio is computed for each sample using the zero standard (Zero). 2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. 3. The %CV and Standard Deviation are calculated for each replicated sample.

Detection of human G-CSF using HTRF (an alternative to ELISA) Cisbio kit 62HGCSFPEG. Also known as CSF3 (colony-stimulating factor 3), G-CSF is a hematopoietic cytokine. It acts on precursor cells in the bone marrow to initiate their proliferation and differentiation into mature granulocytes, on hematopoietic stem cells to induce their mobilization in the blood stream, and on neurons to promote neurogenesis and block apoptosis. In myocardial infarction or ischemia, G-CSF promotes T cells' immune tolerance and tissue recovery. The absence of a functional G-CSF receptor is associated with congenital neutropenia. Cisbio's Human G-CSF kit is a homogeneous, add and read assay to measure G-CSF secretion in supernatant. It offers a fast, efficient alternative to ELISA. This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows: 1. Delta Ratio is computed for each sample using the zero standard (Zero). 2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. 3. The %CV and Standard Deviation are calculated for each replicated sample.