Potency with Parallel Line Equivalence Testing

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Potency calculation of samples within a defined equivalence acceptance range using 4PL analysis.

This analysis performs an unconstrained Four Parameter Logistic (4PL) curve fit on each sample. The determined A, B and D parameters of each unknown’s fit are compared against the reference sample. If these parameter values are within the specified acceptance range the unknown passes for “equivalence” and the IC50 potency and relative potency results are reported.

Samples with parameter values outside of this range are highlighted in red and marked as failed for equivalence.



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After reviewing other analysis parameters press the Calculate button below to process your results.



Dose Dilutions


Acceptance Criteria


Sample IDs


Run Notes