Quantitative analysis of samples using point-to-point.
This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit).
The standard data points (concentration vs. measurement) are plotted on linear axes and point-to-point is used to calculate the concentrations of the samples with any specified dilution factors applied.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.
Samples outside the range of the standards or the fit (greater than the upper asymptote or below than the lower asymptote) are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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