For the quantitative determination of Glucose in serum, plasma, and urine in accordance with Cayman Chemical's Glucose Colorimetric Assay Kit (Item No. 10009582).
This method performs the following calculations.
1. Samples are corrected using the raw value of the zero standard (both Standard and Unknown samples).
2. The corrected absorbance values of each standard are plotted as a function of the glucose concentration, and a linear regression fit method is applied.
3. The concentration of glucose for each sample is calculated using the standard curve fit equation and multiplied by any entered dilution factors.
4. Percentage Coefficient of Variation is calculated for sample replicates.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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