The Thermo Scientific Human IL-1a ELISA Kit measures human IL-1a in culture supernatants, plasma (EDTA, heparin and sodium citrate), serum and urine. This method plots a standard curve on linear axes. This curve is constructed by plotting the known concentrations (X-axis) vs. the average absorbance for each standard (Y-axis). A Four Parameter Logistic curve is fitted through the data points and used to interpolate concentrations of the unknowns. If samples have been diluted prior to the assay, the concentration values are be multiplied by the respective dilution factors. Please provide your absorbance measurements for each well. If measurements were taken at both 450nm and 550nm then please provide the corrected measurements only (i.e subtract 550nm values from 450nm).

The Thermo Scientific Rat IL-1ß ELISA Kit measures human IL-1ß in culture supernatants, plasma (EDTA, heparin and sodium citrate), serum and urine. This method plots a standard curve on linear axes. This curve is constructed by plotting the known concentrations (X-axis) vs. the average absorbance for each standard (Y-axis). A Four Parameter Logistic curve is fitted through the data points and used to interpolate concentrations of the unknowns. If samples have been diluted prior to the assay, the concentration values are be multiplied by the respective dilution factors. Please provide your absorbance measurements for each well. If measurements were taken at both 450nm and 550nm then please provide the corrected measurements only (i.e subtract 550nm values from 450nm).

The Thermo Scientific ELISA Kit is for measuring porcine TNFa in serum; EDTA, heparin and sodium citrate plasma; and culture supernatants. This method plots a standard curve on linear axes. This curve is constructed by plotting the known concentrations (X-axis) vs. the average absorbance for each standard (Y-axis). A Four Parameter Logistic curve is fitted through the data points and used to interpolate concentrations of the unknowns. If samples have been diluted prior to the assay, the concentration values are be multiplied by the respective dilution factors. Please provide your absorbance measurements for each well. If measurements were taken at both 450nm and 550nm then please provide the corrected measurements only (i.e subtract 550nm values from 450nm).

Quantitative analysis of samples using a Four Parameter Logistic (4PL) curve fit suitable for calculating concentrations in accordance with the Thermo Scientific Modified Lowry Protein Assay Kit. This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit). The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.