1 assay found tagged with TRF

Time-Resolved Fluorescence Linear Regression

Quantitative analysis of samples using linear regression on Time-Resolved Fluorescence (TRF) counts. This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the counts (the corrected counts are then used in the fit). The standard data points (concentration vs. measurement) are plotted on semi-log axes and linear regression is performed. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.