ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of corticosterone in biological fluids. This method calculates concentrations in accordance with the Oxford Biomedical Research kit. The data analysis first subtracts the average of the substrate background (blank) from all absorbance values. Next, %B/B0 is calculated for each sample. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4-parameter logistic curve (4PL) is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of isoprostane in urine samples. This method calculates concentrations in accordance with the Oxford Biomedical Research kit. The data analysis first subtracts the average of the substrate background (blank) from all absorbance values. Next, %B/B0 is calculated for each sample. The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4-parameter logistic curve (4PL) is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.