Radioimmunoassay for the quantitative determination of Rat Insulin in serum, plasma, and other tissue culture media.

Enzyme immunoassay (EIA) for the quantification of insulin in mouse and rat sera. A dose response curve of the corrected absorbance (450nm - 590nm) vs. concentration is generated using a 5-parameter logistic fit. Concentrations of insulin, present in the samples, are determined directly from this curve with any specified dilution factors applied.