Quantitative determination of Adrenocorticotropic hormone ACTH in human, mouse and rat plasma samples. This method performs a Four Parameter Logistic (4PL) curve in accordance with MD Bioproducts ACTH ELISA kit for analysis at 450nm (5 calibrators). The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

Quantitative determination of Adrenocorticotropic hormone ACTH in human, mouse and rat plasma samples. This method performs a Four Parameter Logistic (4PL) curve in accordance with MD Bioproducts ACTH ELISA kit for analysis at 405nm (4 calibrators). The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.