Quantitative analysis of samples using a Five Parameter Logistic (5PL) curve fit suitable for calculating concentrations from symmetrical sigmoidal calibrators. The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 5PL is made through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below than the lower asymptote) are highlighted in yellow.