Competitive Enzyme-Linked ImmunoSorbent Assay (ELISA) for the quantification of Microcystin concentration in accordance with EnviroLogix kit (Catalog Number EP 022). This method calculates B/B0% of each sample using the negative control. The standard data points (concentration vs. B/B0%) are plotted on semi-log axes and linear regression is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.