Potency calculation of samples within a defined equivalence acceptance range using 4PL analysis.
This analysis performs an unconstrained Four Parameter Logistic (4PL) curve fit on each sample. The determined A, B and D parameters of each unknown’s fit are compared against the reference sample. If these parameter values are within the specified acceptance range the unknown passes for “equivalence” and the IC50 potency and relative potency results are reported.
Samples with parameter values outside of this range are highlighted in red and marked as failed for equivalence.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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