Enzyme immunoassay (EIA) for the quantification of 11Β Prostaglandin F_2α. This method plots %B/B0 for the standards versus 11Β PGF_2α concentration using linear (y) and log (x) axes and performs a 4-parameter logistic fit. Sample positions with %B/B0 values greater than 80% or less than 20% are highlighted in yellow. These samples should be re-assayed as they generally fall out of the linear range of the standard curve.

Enzyme immunoassay (EIA) for the quantification of 11-dehydro Thromboxane B₂ - Monoclonal. This method plots %B/B0 for the standards versus 11-dehydro TXB₂ concentration using linear (y) and log (x) axes and performs a 4-parameter logistic fit. Sample positions with %B/B0 values greater than 80% or less than 20% are highlighted in yellow. These samples should be re-assayed as they generally fall out of the linear range of the standard curve.

Enzyme immunoassay (EIA) for the quantification of 11-keto Testosterone. This method plots %B/B0 for the standards versus 11KT concentration using linear (y) and log (x) axes and performs a 4-parameter logistic fit. Sample positions with %B/B0 values greater than 80% or less than 20% are highlighted in yellow. These samples should be re-assayed as they generally fall out of the linear range of the standard curve.

Enzyme immunoassay (EIA) for the quantification of 13,14-dihydro-15-keto Prostaglandin F_2a. This method plots %B/B0 for the standards versus 13,14-dihydro-15-keto Prostaglandin F_2a concentration using linear (y) and log (x) axes and performs a 4-parameter logistic fit. Sample positions with %B/B0 values greater than 80% or less than 20% are highlighted in yellow. These samples should be re-assayed as they generally fall out of the linear range of the standard curve.

Enzyme immunoassay (EIA) for the quantification of 15(S)-HETE. This method plots %B/B0 for the standards versus 15(S)-HETE concentration using linear (y) and log (x) axes and performs a 4-parameter logistic fit. Sample positions with %B/B0 values greater than 80% or less than 20% are highlighted in yellow. These samples should be re-assayed as they generally fall out of the linear range of the standard curve.

Competitive immunoassay for the quantitative determination of 17ß-Estradiol in biological and environmental samples. This method plots %B/B0 for the standards versus 17ß-Estradiol concentration using linear (y) and log (x) axes and performs a 4-parameter logistic fit. Concentrations of samples (pg/mL) are determined from the fit and any specified dilution factors are applied.

Quantitative EIA for the determination of 25-hydroxyvitamin D (25-OH D) and other hydroxylated metabolites in human serum or plasma. This method calculates the percentage binding B/B0% of each sample from the zero calibrator (Standard1). A calibration curve is prepared on semi-log axes plotting B/B0% against concentration values with a Four Parameter Logistic curve (4PL) fitted through the data. The the nmol/L is calculated for each sample with specified dilution factor values applied. Supply your absorbance data as reference corrected (i.e. measured at 450nm with reference measured at 650nm).

Competitive protein binding assay for the quantification of 25-OH Vitamin D. A dose response curve of the blank corrected absorbance unit vs. concentration is generated using a 4-parameter logistic fit. Concentrations of 25-OH Vitamin D, present in the samples, are determined directly from this curve with any specified dilution factors applied.

Quantitative analysis of samples using 3rd order polynomial regression. All samples are first corrected by the mean of the blank group measurements. The standard data points are plotted (concentration vs. corrected measurement) and 3rd order polynomial regression is applied to these points. The concentrations of the unknown samples are determined from the fit. To avoid ambiguous results (which can occur from turning points) extrapolation is not used with this method, i.e. concentration values will only be found within the range of the standards.

Enzyme immunoassay (EIA) for the quantification of 6-Keto PGF₁a. This method plots %B/B0 for the standards versus 6-Keto PGF₁a concentration using linear (y) and log (x) axes and performs a 4-parameter logistic fit. Sample positions with %B/B0 values greater than 80% or less than 20% are highlighted in yellow. These samples should be re-assayed as they generally fall out of the linear range of the standard curve.