Quantitative analysis of samples using a Four Parameter Logistic Fit (4PL) suitable for symmetrical sigmoidal data.
All samples are first corrected by the mean of the blank group measurements. The standard data points are plotted (concentration vs. corrected measurement) and a Four Parameter Logistic Fit (4PL) is made through these points. The concentrations of the unknown samples are determined from the fit. It is important to note that concentrations can only be determined for samples which fall within the range of the determined upper and lower asymptotes of the fit (the a and d parameters).
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart. One alternative is to use a suitably low value, such as 0.01 instead of zero. If you make this change it will affect the fit very slightly but is unlikely to affect your results significantly.
You need to be a member for this function.
Please log in or join now.