This data analysis calculates concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Arbor Assays' PGE2 Enzyme Immunoassay kit (K018-H1, K018-H5, K018-H, K018).
All samples are corrected by the mean of the NSB group measurements. The standard data points are plotted (concentration vs. %B/B0) on semi-log axes and a 4PL curve is fitted through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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