The DetectX® Corticosterone Immunoassay kit is designed to quantitatively measure Corticosterone present in extracted dried fecal samples, serum, plasma and tissue culture media samples.
Data analysis is performed in accordance with Arbor Assays' DetectX Corticosterone kit K014-H1, K014-H5, K014-H, K014. Data should be measured with a colorimetric 96 well microplate reader measuring Optical Densities (OD) at 450nm.
All samples are corrected by the mean of the NSB group measurements. The standard data points are plotted (concentration vs. B/B0%) and a Four Parameter Logistic Fit is made through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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